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| FIND Technology ： ウィキペディア英語版 |
FIND Technology
FIND® technology is a directed evolution technology that uses DNA recombination to improve properties of proteins. It eliminates unimportant and deleterious mutations while maintaining and combining beneficial mutations that would enhance protein function.
==Procedures==
For the relevant gene, a library of single stranded oligonucleotides is acquired and then mutated using random mutagenesis. The newly mutated library is then subjected to exonuclease activity, creating both sense and anti-sense fragments. The areas of partially overlapping fragments are then combined and extended using a PCR-like method. These double stranded mutants are then screened for the desired optimized function using a relevant assay. The best mutants are chosen for further exonuclease activity. The process (exonuclease, PCR-like recombination, and mutant screening) is repeated, usually about 10-12 times, in order to achieve the best possible mutants with only beneficial mutations.
抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』
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